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      人轉化生長因子β1(TGFb1)酶聯免疫吸附檢測試劑盒
      ELK1185
      規格: 價格:
      48T ¥1680.00
      96T ¥2400.00

      Overview 文獻

      Product name: Human TGFb1(Transforming Growth Factor Beta 1) ELISA Kit
      Reactivity: Human
      Alternative Names: TGF Beta 1; TGF-B1; CED; DPD1; LAP; Camurati-Engelmann Disease; Latency-associated peptide
      Assay Type: Sandwich
      Sensitivity: 5.8 pg/mL
      Standard: 1000 pg/mL
      Detection Range: 15.63-1000 pg/mL
      Sample Type: serum, platelet-poor plasma, tissue homogenates, cell culture supernates and other biological fluids
      Assay Length: 3.5h
      Research Area: Cytokine;Tumor immunity;Infection immunity;
      Uniprot ID: P01137
      Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TGFb1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TGFb1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TGFb1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TGFb1 in the samples is then determined by comparing the OD of the samples to the standard curve.

      標準曲線

      Concentration (pg/mL) OD Corrected OD
      1000.00 1.950 1.861
      500.00 1.631 1.542
      250.00 1.139 1.050
      125.00 0.815 0.726
      62.50 0.519 0.430
      31.25 0.372 0.283
      15.63 0.226 0.137
      0.00 0.089 0.000

      精密度

      Intra-assay Precision (Precision within an assay):CV%<8%

      Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

      Inter-assay Precision (Precision between assays):CV%<10%

      Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

      回收率

      Matrices listed below were spiked with certain level of recombinant TGFb1 and the recovery rates were calculated by comparing the measured value to the expected amount of TGFb1 in samples.
      Matrix Recovery range Average
      serum(n=5) 81-93% 87%
      EDTA plasma(n=5) 82-94% 88%
      Heparin plasma(n=5) 85-97% 91%

      線性

      The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TGFb1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
      Matrix 1:2 1:4 1:8 1:16
      serum(n=5) 86-93% 91-105% 87-98% 99-102%
      EDTA plasma(n=5) 89-99% 83-101% 93-104% 96-101%
      Heparin plasma(n=5) 85-107% 84-98% 95-103% 86-98%
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